Plant Lipid Signaling Protocols

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Humana Press Plant Lipid Signaling Protocols
Humana Press - Plant Lipid Signaling Protocols

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Beschreibung

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In 
Plant Lipid Signaling Protocols,
 expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied.  These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, 
Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.

Weitere Informationen

Anmerkung Illustrationen:
22 schwarz-weiße und 15 farbige Abbildungen, 13 schwarz-weiße Tabellen
Inhaltsverzeichnis:
Part I: Lipid Analyses1. Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLCTeun Munnik & Xavier Zarza  2. Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLCTeun Munnik 3. Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas ChromatographyMareike Heilmann and Ingo Heilmann 4. Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding AssayIngo Heilmann and Imara Y. Perera 5. Quantification of Diacylglycerol by Mass SpectrometryKatharina vom Dorp, Isabel Dombrink and Peter Dörmann 6. Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGKSteven A. Arisz & Teun Munnik 7. Use of Phospholipase A2 for the Production of Lysophospholipids Steven A. Arisz & Teun Munnik 8. Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas ChromatographyVera Wewer, Peter Dörmann & Georg Hölzl 9. Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass SpectrometrySunitha Shiva, Hieu Sy Vu, Mary R. Roth, Zhenguo Zhou, Shantan Reddy Marepally, Daya Sagar Nune, Gerald H. Lushington, Mahesh Visvanathan, and Ruth Welti 10. Detection and Quantification of Plant Sphingolipids by LC-MS        Jonathan E. Markham 11. Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic AcidNadja Stingl, Markus Krischke, Agnes Fekete  and Martin J. Mueller 12. Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in PlantsSang-Chul Kim, Lionel Faure, and Kent D. Chapman 13. Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMRZachary T. Graber, and Edgar E. Kooijman  Part II: Enzyme Activities14. Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant PeptidesSylvie Collin and Françoise Cochet 15. Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase AssaysYang Ju Im, Irena Brglez, Catherine Dieck, Imara Perera and Wendy F. Boss 16. Assaying Inositol and Phosphoinositide Phosphatase Enzymes Janet L. Donahue, Mustafa Ercetin and Glenda E. Gillaspy 17.  Determination of Phospholipase C Activity In VitroS.M. Teresa Hernández-Sotomayor and  J. Armando Muñoz-Sanchez 18. Assaying Non-Specific Phospholipase C (NPC) ActivityPřemysl Pejchar, Günther F. E. Scherer and Jan Martinec 19. Assaying Different Types of Plant Phospholipase D Activities In Vitro Kirk L. Pappan and Xuemin Wang 20. Measuring PLD Activity In VivoTeun Munnik & Ana M. Laxalt   21. Assaying Plant Phosphatidic Acid Phosphatase ActivityYuki Nakamura 22. Assay of Phospholipase A ActivityMichael Heinze and  Werner RoosJonathan E. Markham Part III: Protein-Lipid Intractions23. Lipid-Binding Analysis using a Fat Blot AssayTeun Munnik and Magdalena Wierzchowiecka 24. Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein InteractionsMagdalena M. Julkowska, Johanna M. Rankenberg and Christa Testerink 25. Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding PropertiesFionn McLoughlin & Christa Testerink Part IV:Imaging26. Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living PlantsJoop E.M. Vermeer and Teun Munnik 27. Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal MicroscopySarah J. Swanson and Simon Gilroy
Herausgeber:
Munnik, Teun;Munnik
Heilmann, Ingo;Heilmann
Bemerkungen:
Presents timely and important tools needed to elucidate particular roles of lipid (-derived) signals in plants


Includes easy-to-use, reproducible protocols


Contains expert tips on troubleshooting and avoiding known pitfalls


Includes supplementary material: sn.pub/extras

Medientyp:
Buch gebunden
Verlag:
Humana Press
Sprache:
Englisch
Auflage:
2013
Seitenanzahl:
305
Zusammenfassung:
This book presents timely and important tools needed to elucidate particular roles of phosphoinositides in plants. It includes easy-to-use, reproducible protocols and contains expert tips on troubleshooting and avoiding known pitfalls.

Stammdaten

Produkttyp :
Buch Gebunden
Verpackungsabmessungen:
0.246 x 0.178 x 0.022 m; 0.72 kg
GTIN:
09781627034005
DUIN:
CL6JGIKMCP5
€ 135,66
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